Application of high-throughput tissue grinder in experiment of young grape leaves
Animal and plant tissue samples are often tested in the laboratory, and the samples are often pre-processed before testing. Grinding is a very critical step in the pre-processing work. The fineness and uniformity of the grinding will affect the subsequent Experimental results. The samples of animal and plant tissues are usually soft in texture, have a certain degree of toughness, and have a small sample size. Conventional knife grinders and planetary ball mills have certain defects when grinding such samples, so is there any special purpose for this type of sample? What about the sample grinder? It is a high-throughput tissue grinder.
The grinding container of the high-throughput tissue grinder is generally a special centrifuge tube. After the grinding is completed, it can be placed directly on the centrifuge for centrifugation. Among them, high-throughput is an adjective, which refers to a large amount of flux, and there are multiple grinding centrifuge tubes.
The high-throughput tissue grinder can realize the grinding, mixing, homogenization and cell disruption of tissue samples. It can grind hard, soft and elastic samples, as well as cell disruption and DNA/RNA extraction. The grinding method can be dry or wet. Grinding and freezing grinding, instruments are used in laboratories in agricultural food, biomedicine, geochemical, environmental quality inspection and other industries.
Experimental steps for grinding young grape leaves:
1. Use a hole puncher with a diameter of 6-8mm to drill holes in the appropriate parts of the blades, and take 8 leaf discs as grinding samples.
2. Put 5 grinding beads with a diameter of 3mm and leaf discs together in a 2ml centrifuge tube (EP tube).
3. Put the centrifuge tube into the adapter, pour liquid nitrogen into the centrifuge tube for freezing, after three to five minutes, close the lid of the centrifuge tube, and wait for another three minutes.
4. Assemble the adaptor into a high-throughput tissue grinder and grind it for three minutes under the condition of 1500 times per minute to obtain finely ground leaf powder, which can be used to extract nucleic acid, protein or carbohydrates.
With woody plants as research materials, it is necessary to extract nucleic acids, proteins or carbohydrates for experimental projects such as genetic or physiological and biochemical analysis research, such as molecular marker research on various fruit trees.